The optimization of a GCxGC system requires a
complex approach since the separation in both columns is
differently and independently influenced by column
dimensions and stationary phases, temperature
programming and carrier gas flow rates. In GCxGC, the
columns of a set are combined in series and the
possibility to apply a close-to-optimal flow regime in
both GCxGC dimensions by columns with a conventional ID
is discussed (see Posters).
The combination of homologous diameter columns, differing in stationary phase and film thickness, was shown to enable each chromatographic dimension to work with a close to the optimal flow regime resulting in an improved phase selectivity (confirmed by system orthogonality estimation) that partially compensated the loss of efficiency due to wider 2D ID.
A new open tubular capillary column called DN-UNIQUE or MEGA-2D is coated in series with different film thickness of two different stationary phases in a single fused silica tubing. This column improves GCxGC performance since it avoids the use of unions (press-fits or low dead volume connections) between the first and the second dimension thus eliminating a possible source of leaks and reducing band broadening effects.
|AN0142||Essential Oils, Quantitative Profiling (GCx2GC-MS/FID)|
|P004||Gas Chromatography and GCxGC|
|P005||Comprehensive two-dimensional GCxGC with conventional inner diameter columns: method development and flow regime optimization|
|P006||Comprehensive two-dimensional GCxGC with conventional inner diameter columns: new column coated with two in series different stationary phases in a single fused silica tubing|
|P007||A new column
coated with two in series different stationary
phases in a single fused
silica tubing of conventional inner diameter for Comprehensive two-dimensional GCxGC